This thesis comprises three chapters focused on fish farming in biofloc systems (BFT), with an emphasis on the feeding management of juvenile Nile tilapia (Oreochromis niloticus). The main objective was to evaluate the contribution of microbial aggregates under conditions of inert feed restriction. Chapter 1 presents a comprehensive review of fish cultivation in BFT systems, highlighting key management parameters that directly affect fish performance, particularly the utilization of microbial aggregates as a nutritional source. Chapter 2 explores the effects of total inert feed restriction over multiple weeks, aiming to assess the capacity of bioflocs to sustain fish growth. Chapter 3 investigates the zootechnical performance of fish under alternating feeding cycles combining feed restriction and provision. Experimental trials were conducted in static biofloc systems composed of isolated experimental units to evaluate the influence of different feeding strategies on solids concentration, nutritional quality of the bioflocs, and microbial community composition. During feeding periods, fish were offered commercial feed three times daily (ad libitum at 9:00 a.m., 1:00 p.m., and 5:00 p.m.). Fish were assessed for performance, body condition, and chemical composition, while hematological parameters were also analyzed in Chapter 2. Water quality was continuously monitored and maintained within optimal ranges for the species. Bioflocs were significantly influenced by the applied feeding strategies, and their nutritional composition affected fish compensatory growth capacity. Full compensation was observed under total feed restriction for up to one week and under partial restriction in 1-day restriction/6-day feeding cycles. Findings indicate that bioflocs play a crucial role in juvenile tilapia feeding management, enhancing feed efficiency, reducing feed costs, and maintaining strategic juvenile stocks to meet market demands, particularly in southern and southeastern Brazil.