Development, characterization and experimental infection of anisakids (Anisakidae: Nematoda) in laboratory

Author: Viviane Barquete Garcia Costa (Currículo Lattes)
Supervisor: Dr Joaber Pereira Junior
Co-supervisor: Dr José Guilherme Bersano Filho


In order to reproduce part of the nematode life cycle in the laboratory, helminths were collected from the pro-ventricle of a specimen of Bobo-Pequeno (Puffinus puffinus). This bird was found dead on the beach, south coast of Brazil. Contracaecum pelagicum eggs were incubated in salted water at 21 ° C, and after 60 h they started hatching. Twenty-four hours after the onset of hatching, 96.3% of the eggs had hatched with larvae at the L3 stage. A significant difference was found in the total length of the larvae between the time of hatching and 60 hours of life. Contracaecum spiculigerum eggs were obtained from a specimen of Magellanic penguin (Spheniscus magellanicus), found dead on the beach. Copepods Acartia tonsa created in the laboratory were exposed to eggs and / or larvae of these nematodes, and were infected. The L3 larvae of C.spiculigerum lodged in the hemocele of these crustaceans. During seven days of the experiment, a significant difference was found in the total length of the larvae removed from the copepods, between day 1 and day 7 post-infection. The larvae no longer had the external cuticle (“cover”) of the L2 stage. The larvae of C. spiculigerum and C. pelagicum hatched in L3, which differs from that observed in other studies, suggesting that the larvae of anisakids emerged from the egg in L2. Two-year-old Paralichthys orbignyanus sole, reared in the laboratory, were inoculated with a solution containing larvae in L3 of C. pelagicum without success in the infection. The results obtained in this work suggest the need for further studies to develop preventive management strategies,contributing to the valorization of the final aquaculture product and the benefits to public health.