Effects of enzyme supplementation on the diet of juvenile Pompano Trachinotus marginatus(Cuvier, 1832)
There is a growing use of vegetable ingredients to replace fishmeal, however its digestion and absorption are hampered by the presence of anti-nutritional factors, such as the structural fibers that make up the vegetable cell wall and phytic acid. The inclusion of exogenous enzymes in diets is an alternative to break the chains of carbonic bonds of plant ingredients, making them available for digestion and absorption, obtaining better use of plant nutrients. Thus, the objective of the present work was to evaluate the effects of the inclusion of an enzyme complex of carbohydrases, proteases and phytase on the digestion and absorption of nutrients, growth performance and possible morphological changes in the liver and intestine of juveniles of silver pampo, Trachinotus marginatus.The experiment was based on the offer of six diets with increasing levels of the enzyme complex Rovabio® Max AP (0, 25, 50, 100, 250 and 500 mg kg-1). 270 juvenile pampo fish (1.0 ± 0.1 g) were stored in 18 40 L tanks. Feed was offered four times a day, until apparent satiety, for 80 days. The results of the experiment showed that the fish from the treatments with 100, 250 and 500 mg of enzymes obtained greater growth performance between treatments. It was observed, in histological sections of the liver, greater lipid deposition in fish from treatments T250 and T500. No intestinal morphological alteration was observed among fish from different treatments. The biochemical profile of the liver showed no significant difference for the glycogen concentration between treatments,while the triglyceride level was significantly higher in the T250 and T500 treatments. The present study demonstrates that supplementation of 100 mg of enzymes is the most suitable enzyme level for diets of juvenile silver pampo, providing improvement in growth performance and little change in liver morphology.